• peripheral blood
• bone marrow aspirates
• cell cultures
• dissociated solid tissue samples, including those frozen or previously embedded

Measurable parameters for high speed analysis include:

Dna histogram demonstrating cell cycle analysis with apoptosis.

• cell or particle size
• cytoplasmic granularity
• fluorescence emission in four different fluorescent bands

Common applications include:cell classification

• qualitative/quantitative analysis of cell surface and cytoplasmic antigens,
• receptors, and glycoconjugates
• cell cycle analysis
• DNA/RNA content and ploidy analysis
• cell viability analysis
• cell purification/separation by physical sorting of living or fixed cells

The flow cytometry lab provides analysis using two types of instrumentation. One is a Becton Dickinson FACSort equipped with an air-cooled argon laser producing fluorescent excitation at 488 nm and featuring a mechanical cell sorting system. The other is a Becton Dickinson FACSVantage SE equipped with a Coherent Enterprise laser producing both 488nm and ultra-violet excitation and featuring an advanced electrostatic sorting system capable of simultaneously sorting two cell populations at the rate of 30,000 cells/second.

For more information, contact Sara Spangenberger at (401) 444-4245 or Paul Monfils at (401) 444-3816.