Djuro Josic, PhD

Director proteomics core heading

Djuro Josic, PhD
COBRE CCRD Proteomics Core Director
Professor of Medicine (Research)

Djuro Josic, PhD, is a professor of medicine and pathology at Brown University and director of the proteomic core in the COBRE Center for Cancer Research Development. He received his research training at Free University in Berlin, Germany and the National Institutes of Health in Bethesda, Maryland. Josic is internationally known for his expertise in the separation, isolation and identification of biopolymers, particularly membrane and plasma proteins. He has substantial practical experience with and knowledge of a large variety of analytical and preparative methods in this field including HPLC, 2-D gel electrophoresis, capillary electrophoresis, affinity purification methodologies, mass spectrometry and similar techniques. The author of more than 100 research articles and book chapters, he has reviewed manuscripts for multiple journals and was the chairman of International Symposium on the Separation of Proteins, Peptides and Polynucleotides (ISPPP'2000) and a member of Scientific Committee of ISPPP 1998, 2002 and 2004.

Research focus
Josic's scientific interest is focused on two major topics:

Purification and isolation of membrane and membrane associated proteins differentially expressed in normal and malignant cells. His current work is focused on characterization of annexins, a family of calcium binding membrane associated proteins found in the liver and hepatocellular carcinomas.

Development of new pharmaceuticals based on human plasma. During his work in industry, Josic was involved in development of several new therapeutics including new preparations of clotting factor VIII, clotting factor IX and antithrombin III. Together with Yow-Pin Lim, MD and Douglas Hixson, PhD, Josic is currently involved in development of virus and immunologically safe preparation of inter-alpha inhibitor proteins from human plasma for treatment of sepsis and other inflammatory diseases.

Selected Publications
Josic Dj and Zeilinger K, Membrane proteins, Methods in Enzymology (1996) 271: 113-134.

Strancar A, Koselj P, Schwinn H and Josic Dj, Application of compact, porous disks for fast separation of biopolymers and in-process control in biotechnology, Analytical Chemistry (1996) 68: 3483-3488.

Josic Dj, Kannicht Ch, Loester K, Pock K, Iberer G and Buchacher A, Vitronectin in clotting factor IX concentrates, Haemophilia (2001) 7: 250-257.

Sanko E, Sarafanov A, Ananyeva N, Behre E, Shima M, Schwinn H and Josic Dj, Comparison of the properties of phospholipids surfaces formed on HPA and L1 biosensor chips for binding of coagulation factor VIII, Journal of Chromatography A (2001) 921: 49-57.

Yang S, Lim Y-P, Zhou M, Salvemini P, Schwinn H, Josic Dj, Koo DJ, Chaudra IH and Wang P, Administration of human inter-a-inhibitors maintains hemodynamic stability and improves survival during sepsis, Critical Care Medicine (2002) 30: 617-622.

Saenko E, Kannicht Ch, Loester K, Sarafanov A, Khrenov A, Kouiavskaia D, Shima M, Anayeva N, Schwinn H, Gruber G and Josic Dj, Development and applications of surface plasmon resonance-based von Willebrand factor-collagen binding assay, Analytical Biochemistry (2002) 302: 252-262.

Branovic K, Buchacher A, Barut M, Strancar A and Josic Dj, Application of semi-industrial monolithic columns for downstream processing of clotting factor IX, Journal of Chromatography B (2003) 790: 175-182.

Demelbauer UM, Plematl A, Kremser L, Allmaier G, Josic Dj and Rizzi A, Characterization of glyco isoforms of plasma derived human antithrombin by on-line CZE-ESI-QIT-mass spectrometry of intact proteins, Electrophoresis (2004) accepted for publication.

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