Immunohistochemistry Specialty Testing
The Molecular Core laboratory's Ventana Discovery is a totally automated staining module that uses bar code technology. The Discoveryoffers walk-away automation, reliable reproducibility, flexibility and data management, as well as total automation in the areas of IHC staining of frozen or paraffin embedded sections of animal or human tissue, FISH and ISH using DNA, RNA or oligonucleotide probes on sections or microarrays (gene expression).
Immunohistochemistry
- Non-solvent based removal of paraffin
- Automated epitope unmasking
- Flexible primary antibody incubation options
- Four available detection chemistries
- DAB, AEC, Fast Red, NBT
- Automated counterstaining
In Situ Hybridization
- Rehydration of slides
- Promote uniform spreading/reduce nonspecific hybridation
- Denaturation
- Hybridization
- Stringency washes
- Optional secondary detection
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DNA Microarray
- Target unmasking
- Automated probe application
- Selectable time and temperature denaturation
- Selectable time and temperature hybridization
- Selectable time, stringency and temperature washes
- Detection counterstain
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Photos and charts shown courtesy of Ventana Discovery.
One Hoppin Street • Providence, RI 02903
Phone: 401-444-7237 • email: AGarcia3@lifespan.org |
