Laser Capture Microdissection

LCM offers a method for procuring pure cells from specific microscopic regions of tissue sections and which allows the application of molecular analysis methods to study evolving disease lesions in actual tissue.

The benefits of the lab's AutoPix LCM are multiple slide handling, versatile capture modes (manual, semimanual and automated), rapid capture of large numbers of cells, image inventory software, very easy software controls, robust instrumentation and robotics and low energy infrared laser that does not damage cells.

	Automated, versatile andself-contained Laser Capture Microdissection (LCM) system The completely enclosed instrument minimizes contamination and sensitively detects fluorescent samples. The high-intensity fluorescence illuminator has three filer cubes for red, green and blue fluorescence.
	The Sample Station Three different sample slides can be dissected at a single session. The cap tray holds eight caps, either HS or Macro. Used caps containing captured cells are robotically moved to the QC station for evaluation and then to the removeable unload station for retrieval. DNA, RNA or protein is then extracted using reagent kits available from Arcturus.

Visit the Arcturus for more information about the Arcturus AutoPix Laser Capture Microdissection system. This web site has all of the information you need to get started. Please read the two application notes before contacting us to use the instrument. Photos and charts shown courtesy of Arcturus.

Laser Capture Microdissection (LCM) Systems

Application Notes

Tissue Scrape Protocol for Verifying RNA Quality Using the PicoPure™ RNA Isolation Kit

Optimized Protocol for Preparing and Staining LCM Samples from Frozen Tissue and Extraction of High-Quality RNA

Supplies You Will Need

CapSure Caps for LCM

PicoPure™ RNA Isolation Kit

RiboAmp® OA RNA Amplification Kit

HistoGene™ LCM Frozen Section Staining Kit

Other Useful Links

LCM at NICHD

LCM at NIEHS

NCI/CIT Molecular Profiling Initiative

Other useful links can be found on the Arcturus website.

One Hoppin Street • Providence, RI 02903
Phone: 401-444-7237 • email: AGarcia3@lifespan.org