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Quantitative Real-Time PCR by Mx4000 from StratageneQuantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables. The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses. The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction. The Mx4000 for Real-time PCR
The Optics
Applications
Visit Stratagene's website for more information about the MX4000 real-time PCR system. Photos and charts shown courtesy of Stratagene. Other Useful Links
Oligo Houses One Hoppin Street • Providence, RI 02903 |
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