James Clifton, PhD
Research Associate

James Clifton, PhD, is a research associate in the COBRE CCRD proteomics core. He earned his doctorate degree at Yale University in New Haven, Connecticut and continued his research training at Brandeis University in Waltham, Massachusetts and Boston Children's Hospital where he held the rank of instructor at Harvard University.

He has extensive practical experience in the field of protein biochemistry, including the over-expression of proteins using recombinant DNA technology; the isolation of both soluble and membrane proteins at analytical (sub-microgram) and preparative (multi-milligram) scales; and the purification of proteins, using techniques such as gel electrophoresis, affinity methodologies, and liquid chromatography.

Selected Publications
Kallarakal AT, Mitra B, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL. (1995), Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the K166R mutant, Biochemistry 34:2788-97.

Taylor R, Burgner JW, Clifton J, Cramer WA. (1998), Purification and characterization of monomeric Escherichia coli vitamin B12 receptor with high affinity for colicin E3, J. Biol. Chem. 273:31113-8.

Ondrechen MJ, Clifton JG, Ringe D. (2001), THEMATICS: a simple computational predictor of enzyme function from structure, Proc. Natl. Acad. Sci. USA 98:12473-8.

Beebe JA, Arabshahi A, Clifton JG, Ringe D, Petsko GA, Frey PA. (2003), Galactose mutarotase: pH dependence of enzymatic mutarotation, Biochemistry 42:4414-20.

One Hoppin Street • Providence, RI 02903
Phone: 401-444-7237 • email: AGarcia3@lifespan.org