Cellular and Molecular Neuroscience Core Resources

Cellular and Molecular Neuroscience Core Resources

Core laboratory resources are available to NPNI and BIBS clinical and translational research scientists. Deep Brain 41

  • Equipment and Services Located in Claverick Building, Rooms 405, 407 and 320:
    • Tissue culture incubators;
    • Chemical hoods;
    • Ultracentrifuges;
    • Cold room;
    • Electrophoresis equipment (immuno-electrophoresis, PAGE, analytical and preparative isoelectric focusing equipment);
    • Dark room;
    • Perkin Elmer/Cetus Automated Thermal Cycler and Roche LightCycler 480;
    • MagNApure Polymerase Chain Reaction (PCR) instruments;
    • Analox GL6 Multiassay Analyzer for measuring blood alcohol and lipid chemistry;
    • BioRobot EZ1 for RNA and DNA purification;
    • TissueLyser II for high through-put homogenization up to 48-samples simultaneously (bead mill based);
    • epMotion 5070 automated pipetting system (Eppendorf);
    • McIllwain Tissue Choppers (Stoelting Co) to generate tissue slice cultures from brain and other organs;
    • Stereologer (Stereology Resource Center) computerized system for unbiased 3-D stereology (quantification of total density and numbers of cells, total fiber length and fiber density, total surface area and surface density and spatial distribution of biological objects);
    • Nicolet Biomedical Inc. Viking IV EMG Electromyography System;
    • McIllwain Tissue Choppers (Stoelting Co) for generating tissue slice cultures.
  • Equipment/Resources Located in Molecular Pathology (Aldrich 6, Room 600):
    • Beecher tissue micro-arrayer to construct tissue micro-array libraries from paraffin sections for simultaneous in situ gene and protein expression studies;
    • Arcturus Autopix Laser capture microdissection system;
    • Agilent Bioanalyzer to assess integrity of mRNA and DNA.
  • Equipment/Resources Located in the Proteomics Core and Core Research Laboratory (Coro Building, Room 4.213):
    • Liquid Chromatography-Mass Spectrometry;
    • MALDI Imaging Mass Spectrometry (UltrafleXtreme MALDI-TOF/TOF mass spectrometer equipped with a Smartbeam II laser and the Flex software suite - FlexControl, FlexImaging, and FlexAnalysis - Bruker Daltonics);
    • Automatic and control matrix sprayer ImagePrep system (Bruker Daltonics);
    • Biotools software (Bruker Daltonics) and Mascot algorithm V.2.3.2 (Matrix Science) for peptide or protein sequence database searching;
    • Cryostat (Leica CM3050 S - Leica Microsystems).
  • Services offered by the Core Research Laboratories (Coro Building, Room 4.213)
    • Histology [paraffin embedding, staining];
    • Cryostat sectioning; Custom tissue micro-arrays; Immunohistochemical staining;
    • ELISAs; Multiplex assays of proteins and gene expression; Image analysis; Confocal laser microscopy;
    • Electron microscopy;
    • Fluorescence microscopy and photography;
    • Access to statistical software for data analysis.
  • Services offered by the Department of Pathology (APC Building, 12 Floor):
    • Full range of routine and immunological assays on blood, serum, plasma, CSF, and tissue for investigators who have collected samples for analysis.

Investigators who have designed studies, but have not yet collected samples, should consult with the core laboratory staff for experimental design and optimizing sample preparation.

For more information, including design consultations, scheduling and pricing, contact:

Director: Suzanne De La Monte
Tel: (401) 444-7364
Email: suzanne_delamonte_md@brown.edu

MALDI Tissue and Cellular Mass Spectrometry
Kim Miran and Jared Kay
Tel: (401) 444-4493
Email: Miran_Kim@Brown.edu; Jared_Kay@alumni.brown.edu

Tissue microarrays, immunohistochemistry, ELISAs, Multiplex assays
Leila (Loli) Noble
Tel: (401) 444-3815
Email: LNoble@lifespan.org

Electron Microscopy
Carol Ayala
Tel: (401) 444-3815
Email: Cayala@lifespan.org

Histology, including paraffin and cryosectioning
Paul Monfils
Tel: (401) 444-3815
Email: Pmonfils@lifespan.org

Confocal Microscopy
Virginia Hovanesian
Tel: (401) 444-3815
Email: vhovanesian@lifespan.org