James Mastrostefano, APRN-CNP, is a family nurse practitioner at Jamestown Family Practice, a Lifespan Physician Group practice. He earned his master of science in nursing from Marymount University in Arlington, Virginia, and graduated summa cum laude with a bachelor of science in nursing from the University of Rhode Island. Mastrostefano is a member of Sigma Theta Tau International Honor Society of Nursing and The American Academy of Nurse Practitioners.

Mastrostefano previously practiced as a registered nurse on a medical-surgical and COVID-19 designated unit at Medstar Washington Hospital Center in Washington, DC.