Extracellular Vesicle Core

Contact the Extracellular Vesicle Core 

Sicheng Wen MD, PhD, Director
Email: sicheng _wen@brown.edu
Phone: 401-444-2480

CORO West Research Building
1 Hoppin Street
Suite 5.01
Providence, RI

About Us

The COBRE Center for Stem Cells and Aging (SCA) at Rhode Island Hospital has developed the unique Extracellular Vesicle Core. The aims of the Extracellular Vesicle Core are to provide the research community at Rhode Island Hospital, and researchers outside of Rhode Island Hospital, with our expertise, infrastructure and state-of-the-art EV technologies to support research in the fields of extracellular vesicle research.

Services

The COBRE Extracellular Vesicle Core staff have been successfully providing services to the research community in the Rhode Island area with their expertise in vesicle harvest, isolation, characterization, and function analysis for more than ten years.

Equipment

Ultracentrifuge

Ultracentrifuge

Ultracentrifugation is the classical method for extracellular vesicles isolation biofluid. The Extra Cellular Vesicle Core contains two Thermo Fisher WX80 ultracentrifuges with Sorvall AH-629 rotors.

TangenX

TangenX

By using tangential flow filtration (TFF) technology, TangenX delivers rapid and highly efficient isolation of extracellular vesicles from large volumes of samples.

NanoSight analysis

Nanoparticles are analyzed using the NanoSight NS500 instrument (NanoSight Ltd) with NTA software. This service is under the COBRE Flow Cytometry Core.

ViiA 7 real-time PCR system with twister robot

ViiA PCR system

We identify extracellular vesicles MicroRNA profiling using the ABI TaqMan MicroRNA assay by ViiA 7 Real-Time PCR System. This system allows look up to 758 unique microRNA per sample, quickly and efficiently with as little as 1ng of input total RNA with pre-amplification for a full miRNA profile.


Facility Rates

Service/Instrument

Rate

Initial scientific consultation of EV experimental design Free
Culture human mesenchymal stem cells for EV isolation (not including reagents) $50/ working hour
Isolation of EV from cell culture medium and other biofluids by differential ultracentrifugation Technical assistance/ training: $50/hour
Without technical assistance: $25/hour
Isolation of EV from cell culture medium by tangential flow filtration (not including the TangenX™ Flat Sheet Cassettes) $50/liter medium
Preparation of vesicle-depleted FBS by differential ultracentrifugation $25/hour
EV Electron microscopy (EM) sample preparation $185/run (up to 4 samples)
Immunoblotting for vesicle markers (CD9, CD63 and CD81) $280/run
EV fluorescent labeling $100/ up to 6 samples labeling
Exosome RNA extraction (including QC by nanodrop) $150/ up to 10 samples
mRNA or microRNA expression in EVs by TaqMan Real-Time qPCR array (not including reagents) Technical assistance/ training: $50/hour
Without technical assistance: $25/hour
NanoSight Tracking Analysis (NanoSight 5000) Service under COBRE Flow Cytometry Core

In conjunction with the Flow Cytometry Core at the COBRE Center for Stem Cells and Aging, we also provide other services, including EV uptake analysis by flow cytometry.

The list of service fee is for academic and non-profit investigators. Projects from the COBRE Center for Stem Cells and Aging are offered core services free of charge, and COBRE Center for Stem Cells and Aging investigators receive a 20% discount. For information about rates for other investigators, please contact Dr. Sicheng Wen at 401-444-2480.

Learn more about the COBRE Center for Stem Cells and Aging »