COBRE Center for Stem Cells and Aging

Lentivirus Construct Core

Lentivurs Construct Core srevice flow chart

The Lentivirus Construct Core of the COBRE Center for Stem Cells and Aging (SCA) at Rhode Island Hospital serves investigators from almost every department of the Warren Alpert Medical School of Brown University, many labs in Rhode Island Hospital, Women & Infants Hospital, and the Providence VA Medical Center. 

In response to the COVID-19 pandemic, the Lentivirus Construct Core rapidly developed a series of BSL-2 level SARS-CoV-2 pseudoviruses, including the most recent highly infectious Delta and Omicron variants. These SARS-CoV-2 pseudoviruses have drawn national attention and were requested by COVID19 investigators from around the country. Since then, a number of impactful studies using our SARS-CoV-2 pseudoviruses have been published in Nature Chemical Biology, PLOS Biology, JCI Insight, Frontiers in Immunology, Pharmaceuticals, and Oncotarget. Several Invention Disclosures related to these studies have also been filed. Thus, the Lentivirus Construct Core has made a meaningful contribution to the global effort against the COVID-19 pandemic.

Our Mission

The Lentivirus Construct Core aims to share lentivirus technologies for easy access and efficient use and to enhance the competitiveness of Rhode Island investigators to secure federal research funding.


Key services available include:

  • Lentivirus-mediated stable gene overexpression in cells
  • Lentivirus-mediated CRISPR/Cas9 gene knockout in cells
  • Stably labeling cells with fluorescence protein, luciferase or antibiotic resistance
  • Site-directed mutagenesis
  • Lentivirus and retrovirus packaging

Contact the Lentivirus Construct Core

Core Director: Olin Liang, PhD
Email: [email protected]
Phone: 401-444-5331
Coro West Research Building, Suite 4.200
1 Hoppin St. 
Providence, RI 02903