Flow Cytometry Core
The Rhode Island Hospital Stem Cells and Aging (SCA) COBRE Flow Cytometry Core function is to provide high quality, cost effective, state-of-the-art flow cytometry and multiparameter cell sorting instrumentation and associated expertise and services to all investigators in the SCA COBRE and to the general research community in Rhode Island.
COBRE Flow Cytometry Core Staff and Services
Mark Dooner directs and manages the core and serves as operator for the BD Biosciences Influx cell sorter and LSRII. An advisor is available to the investigators in experimental design and data interpretation.
The core has had experience with a variety of different flow cytometry applications and protocols and will work with both experienced and novice investigators to help plan and execute successful flow cytometry experiments.
|Instrument or Service||
Academic, Per Hour
Industry, Per Hour
|LSRII (self use)||$38.50||$46.20|
|Influx cell sorting||$82.50||$99|
|CyTOF sample preparation||$33||$39.60|
|Offline cytometry analysis||$71.50||$85.80|
All first-time users must contact Mark Dooner to discuss their project and to set up a user account. Every effort will be made to accommodate sorting requests in a timely manner. Booking in advance is recommended.
Please note SCA COBRE projects and pilot projects will be given priority.
Flow Cytometry Core Equipment
BD Biosciences Influx Cell Sorter
The sorter separates particles on the detection of fluorescent markers on particles such as cells, chromosomes, bacteria or nuclei.
Then the collected cells are suitable for downstream analysis either in molecular applications like protein assays and PCR, or used in viable assays such as tissue culture or injected into in vivo models.
Generated data may also be used to determine percentages of interrogated populations. The sorter can separate populations into tubes (1.5ml, 4ml, 15ml, 50ml), multi-well plates (6, 24, 48, 96, 384), or directly onto microscope slides.
BD Biosciences LSRII Analyzer/Flow Cytometer
Fluidigm Helios (CyTOF) Mass Cytometry
Now available through the COBRE Flow Cytometry Core, mass cytometry combines the technologies of flow cytometry with that of mass spectrometry. It applies nebulization, aerosolization and ionization to cells labeled with heavy metal isotope-conjugates, allowing for ion separation based on their mass:charge ratio and quantification of each isotope on a per cell basis ultimately determining properties of a cell population at a single-cell resolution.
The technique of mass cytometry is powerful, as it can analyze more cellular targets without the problem of compensation as seen in flow cytometry. The Helios is capable of simultaneously analyzing up to more than 50 parameters both on cell surface and intracellularly.